Our group is running an ERC project (Starting Grant to A. Loquet) for the period 2015-2020. The project is called Weakinteract, for “Weak interactions in self-organizations studied by NMR spectroscopy in the supramolecular solid-state“. This project is related to the development of solid-state NMR methods to tackle complex supramolecular assemblies in the fields of biology and chemistry.
The current results of this ERC project have been reported in several publications
Structures of Type III Secretion System Needle Filaments.
Habenstein B, El Mammeri N, Tolchard J, Lamon G, Tawani A, Berbon M, Loquet A.
Curr Top Microbiol Immunol. 2020 Jan 24. doi: 10.1007/82_2019_192.
Structural dissection of amyloid aggregates of TDP-43 and its C-terminal fragments TDP-35 and TDP-16.
Shenoy J, El Mammeri N, Dutour A, Berbon M, Saad A, Lends A, Morvan E, Grélard A, Lecomte S, Kauffmann B, Theillet FX, Habenstein B, Loquet A.
FEBS J. 2019 Nov 29. doi: 10.1111/febs.15159.
Structural insights into the AapA1 toxin of Helicobacter pylori.
Korkut DN, Alves ID, Vogel A, Chabas S, Sharma CM, Martinez D, Loquet A, Salgado GF, Darfeuille F.
Biochim Biophys Acta Gen Subj. 2020 Jan;1864(1):129423. doi: 10.1016/j.bbagen.2019.129423.
Molecular architecture of bacterial amyloids in Bacillus biofilms.
El Mammeri N, Hierrezuelo J, Tolchard J, Cámara-Almirón J, Caro-Astorga J, Álvarez-Mena A, Dutour A, Berbon M, Shenoy J, Morvan E, Grélard A, Kauffmann B, Lecomte S, de Vicente A, Habenstein B, Romero D, Loquet A.
FASEB J. 2019 Nov;33(11):12146-12163. doi: 10.1096/fj.201900831R.
A polymorphic helix of a Salmonella needle protein relays signals defining distinct steps in type III secretion.
Guo EZ, Desrosiers DC, Zalesak J, Tolchard J, Berbon M, Habenstein B, Marlovits T, Loquet A, Galán JE.
PLoS Biol. 2019 Jul 1;17(7):e3000351. doi: 10.1371/journal.pbio.3000351.
Structural and Functional Characterization of the Type Three Secretion System (T3SS) Needle of Pseudomonas aeruginosa.
Lombardi C, Tolchard J, Bouillot S, Signor L, Gebus C, Liebl D, Fenel D, Teulon JM, Brock J, Habenstein B, Pellequer JL, Faudry E, Loquet A, Attrée I, Dessen A, Job V.
Front Microbiol. 2019 Mar 29;10:573. doi: 10.3389/fmicb.2019.00573.
Molecular mechanisms for the destabilization of model membranes by islet amyloid polypeptide.
Divakara MB, Martinez D, Ravi A, Bhavana V, Ramana V, Habenstein B, Loquet A, Santosh MS.
Biophys Chem. 2019 Feb;245:34-40. doi: 10.1016/j.bpc.2018.12.002.
1H, 13C, 15N NMR resonance assignments and secondary structure determination of the extra-cellular domain from the human proapoptotic TRAIL-R2 death receptor 5 (DR5-ECD).
Baudin A, Guichard A, Collie GW, Rousseau S, Chaignepain S, Hocquellet A, Berbon M, Loquet A, Mackereth C, Guichard G, Odaert B.
Biomol NMR Assign. 2018 Oct;12(2):309-314. doi: 10.1007/s12104-018-9828-1.
3D structure determination of amyloid fibrils using solid-state NMR spectroscopy.
Loquet A, El Mammeri N, Stanek J, Berbon M, Bardiaux B, Pintacuda G, Habenstein B.
Methods. 2018 Apr 6. pii: S1046-2023(17)30251-7. doi:10.1016/j.ymeth.2018.03.014
Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR.
Tolchard J, Pandey MK, Berbon M, Noubhani A, Saupe SJ, Nishiyama Y, Habenstein B, Loquet A.
J Biomol NMR. 2018 Mar;70(3):177-185. doi: 10.1007/s10858-018-0168-3
Coiled-coil oligomerization controls localization of the plasma membrane REMORINs.
Martinez D, Legrand A, Gronnier J, Decossas M, Gouguet P, Lambert O, Berbon M, Verron L, Grélard A, Germain V, Loquet A, Mongrand S, Habenstein B.
J Struct Biol. 2018 Feb 23. pii: S1047-8477(18)30046-7. doi: 10.1016/j.jsb.2018.02.003.
Diversity of Amyloid Motifs in NLR Signaling in Fungi.
Loquet A, Saupe SJ.
Biomolecules. 2017 Apr 13;7(2). pii: E38. doi: 10.3390/biom7020038
Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy.
Habenstein B, Loquet A.
Methods Mol Biol. 2017;1615:415-448. doi: 10.1007/978-1-4939-7033-9_29.
Structural basis for plant plasma membrane protein dynamics and organization into functional nanodomains.
Gronnier J, Crowet JM, Habenstein B, Nasir MN, Bayle V, Hosy E, Platre MP, Gouguet P, Raffaele S, Martinez D, Grelard A, Loquet A, Simon-Plas F, Gerbeau-Pissot P, Der C, Bayer EM, Jaillais Y, Deleu M, Germain V, Lins L, Mongrand S.
Elife. 2017 Jul 31;6. pii: e26404. doi: 10.7554/eLife.26404
Lipid Internal Dynamics Probed in Nanodiscs.
Martinez D, Decossas M, Kowal J, Frey L, Stahlberg H, Dufourc EJ, Riek R, Habenstein B, Bibow S, Loquet A.
Chemphyschem. 2017 Jun 1. doi: 10.1002/cphc.201700450
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy.
Loquet A, Tolchard J, Berbon M, Martinez D, Habenstein B.
J Vis Exp. 2017 Sep 17;(127). doi: 10.3791/55779.
NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils
Stanek J, Andreas LB, Jaudzems K, Cala D, Lalli D, Bertarello A, Schubeis T, Akopjana I, Kotelovica S, Tars K, Pica A, Leone S, Picone D, Xu ZQ, Dixon NE, Martinez D, Berbon M, El Mammeri N, Noubhani A, Saupe S, Habenstein B, Loquet A, Pintacuda G.
Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15504-15509. doi: 10.1002/anie.201607084
Identification of a novel cell death inducing domain reveals that fungal amyloid-controlled programmed cell death is related to necroptosis
Daskalov A, Habenstein B, Sabaté R, Berbon M, Martinez D, Chaignepain S, Coulary-Salin B, Hofmann K, Loquet A, Saupe SJ
Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2720-5. doi: 10.1073/pnas.1522361113